H2N2V1, 1968, Analysis, bibRecord, 000039

Influenza A Virus Isolation, Culture and Identification

Identifieur interne : 000039 ( 1968/Analysis ); précédent : 000038; suivant : 000040

Influenza A Virus Isolation, Culture and Identification

Auteurs : Amie J. Eisfeld [États-Unis] ; Gabriele Neumann [États-Unis] ; Yoshihiro Kawaoka [États-Unis, Japon]

Source :

Nature protocols [ 1754-2189 ] ; 2014.

RBID : PMC:5619698

Abstract

SUMMARY

Influenza A viruses (IAV) cause epidemics and pandemics that result in considerable financial burden and loss of human life. To manage annual IAV epidemics and prepare for future pandemics, improved understanding of how IAVs emerge, transmit, cause disease, and acquire pandemic potential is urgently needed. Fundamental techniques essential for procuring such knowledge are IAV isolation and culture from experimental and surveillance samples. Here, we present a detailed protocol for IAV sample collection and processing, amplification in chicken eggs and mammalian cells, and identification from samples containing unknown pathogens. This protocol is robust, and allows for generation of virus cultures that can be used for downstream analyses. Once experimental or surveillance samples are obtained, virus cultures can be generated and the presence of IAV can be verified in 3–5 days. Increased time-frames may be required for less experienced laboratory personnel, or when large numbers of samples will be processed.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5619698

DOI: 10.1038/nprot.2014.180
PubMed: 25321410
PubMed Central: 5619698

Affiliations:

Links to Exploration step

PMC:5619698

Le document en format XML

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<p id="P1">Influenza A viruses (IAV) cause epidemics and pandemics that result in considerable financial burden and loss of human life. To manage annual IAV epidemics and prepare for future pandemics, improved understanding of how IAVs emerge, transmit, cause disease, and acquire pandemic potential is urgently needed. Fundamental techniques essential for procuring such knowledge are IAV isolation and culture from experimental and surveillance samples. Here, we present a detailed protocol for IAV sample collection and processing, amplification in chicken eggs and mammalian cells, and identification from samples containing unknown pathogens. This protocol is robust, and allows for generation of virus cultures that can be used for downstream analyses. Once experimental or surveillance samples are obtained, virus cultures can be generated and the presence of IAV can be verified in 3–5 days. Increased time-frames may be required for less experienced laboratory personnel, or when large numbers of samples will be processed.</p>
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Influenza A Virus Isolation, Culture and Identification

Influenza A Virus Isolation, Culture and Identification

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